The raw contigs FASTA file, the GTF annotations and the peptide sequences were downloaded from:ftp://ftp.ncbi.nlm.nih.gov/genomes/ The hard-masked FASTA file was produced by Perl conversion of the soft-masked sequence from NCBI with the following: perl -lne 'if(!/^>/){ s/[a-z]/N/g } print' Nicotiana_tabacum.TN90.GCF_000715135.1.NCBI.dna.toplevel.fa > \ Nicotiana_tabacum.TN90.GCF_000715135.1.NCBI.dna_rm.genome.fa The rest of files were produced by RSAT-Tools to install this genome.