CONFIGURATION ============= ... INSTALLING MAF FILES =================== The current version of peak-footpints requires to download the complete set of multiple alignment files (MAF) from the UCSC Genome Browser, for a selected set of species and genome versions. Downloading the MAFs -------------------- - Open a connection to UCSC server (http://genome.ucsc.edu/) - Click on "Downloads" - On the Download page, click on the reference organism (the organism from which the ChIP-seq peaks were obtained e.g. Mouse) - Choose a genome version (e.g. mm8) Under the title "Multiple Alignments", follow the link "Multiple alignments of NN vertebrate genomes with XXX" (e.g. Multiple alignments of 16 vertebrate genomes with Mouse) - Download all the files except those containing the suffix "random.maf.gz" and uncompress them with the command gunzip. Indexing MAFs ------------- - ...