Error: Error with the expected frequency file : the sum must be strictly positive 0
Error occurred on RSAT site: rsat; host server: rsat; admin: compbio@eead.csic.es

; matrix-scan -v 1 -quick -matrix_format transfac -m $RSAT/public_html/tmp/www-data/2026/07/23/matrix-scan_2026-07-23.104512_NaBJTj.matrix -pseudo 1 -decimals 1 -2str -origin end -bginput -markov 1 -bg_pseudo 0.01 -return limits -return sites -lth score 1 -i $RSAT/public_html/tmp/www-data/2026/07/23/tmp_sequence_2026-07-23.104512_q8kdW2.fasta -seq_format fasta -n score ; Quick counting mode ; Input files ; input $RSAT/public_html/tmp/www-data/2026/07/23/tmp_sequence_2026-07-23.104512_q8kdW2.fasta ; Matrix files ; matrix 1 $RSAT/public_html/tmp/www-data/2026/07/23/matrix-scan_2026-07-23.104512_NaBJTj.matrix ; Sequence format fasta ; Pseudo counts 1 ; Background model ; Method input ; Markov order 1 ; Strand undef ; Background pseudo-frequency 0.01 ; Residue probabilities ; a 0.00000 ; c 0.00000 ; g 0.00000 ; t 0.00000 ; WARNING 16 missing transitions. You should reduce the Markov order ; Thresholds lower upper ; score 1 NA ; Output columns ; 1 seq_id ; 2 ft_type ; 3 ft_name ; 4 strand ; 5 start ; 6 end ; 7 sequence ; 8 weight #seq_id ft_type ft_name strand start end sequence weight ; ; Matrices ; matrix name ncol nrow pseudo Wmin Wmax Wrange ; 1 Fis 18 4 0 0.000 0.000 0.000 a:0.000 c:0.000 g:0.000 t:0.000 ; 2 H-NS 12 4 0 -Inf 0.000 Inf a:0.000 c:0.000 g:0.000 t:0.000 ; Number of sequences scanned 0 ; Sum of sequence lengths 0 ; N residues 0 ; Matches per matrix ; matrix name matches scored ; 1 Fis 0 0 ; 2 H-NS 0 0 ; TOTAL 0 0 ; Host name rsat ; Job started 2026-07-23.104512 ; Job done 2026-07-23.104512 ; Seconds 0.15 ; user 0.15 ; system 0.03 ; cuser 0.08 ; csystem 0.03